Journal: International Journal of Molecular Medicine

Article Title: CCN5 attenuates profibrotic phenotypes of fibroblasts through the Smad6-CCN2 pathway: Potential role in epidural fibrosis

doi: 10.3892/ijmm.2015.2190

Figure Lengend Snippet: CCN5 transfection enhances CCN5 expression in primary fibroblasts. Primary fibroblasts were obtained from the tail skin of rats and cultured in DMEM medium. Lentivirus plasmid pWPT-GFP was introduced to construct the recombinant pWPT-CCN5 plasmids, following packaging with vectors of pCMV-VSV-G and pCMV-dR8.91. The corresponding transfection effect of CCN5 mRNA (A) and protein levels (B) was assessed individually with RT-PCR and western blotting. * P<0.05.

Article Snippet: The 293 cells were then co-transfected with the lentivirus plasmids pWPT-CCN5, packaging vectors of pCMV-VSV-G and pCMV-dR8.91 (Clontech, Saint-Germain-en-Laye, France) using Lipofectamine ® 2000 Reagent (Invitrogen-Life Technologies, Carlsbad, CA, USA) at 37°C, 5% CO 2 for 12 h. The cultured medium was collected and filtered.

Techniques: Transfection, Expressing, Cell Culture, Plasmid Preparation, Construct, Recombinant, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Cell chemical biology

Article Title: Blockade of Oncogenic NOTCH1 with the SERCA Inhibitor CAD204520 in T-cell Acute Lymphoblastic Leukemia

doi: 10.1016/j.chembiol.2020.04.002

Figure Lengend Snippet: RESOURCE AVAILABILITY

Article Snippet: pCMV-VSV-G packaging vector , Addgene , Cat# 8454; RRID: Addgene_8454.

Techniques: Plasmid Preparation, Expressing, Recombinant, Modification, DC Protein Assay, ATP Assay, Staining, Software

Journal: Molecular oncology

Article Title: Small Molecule Growth Inhibitors of Human Oncogenic Gammaherpesvirus Infected B-Cells

doi: 10.1016/j.molonc.2014.09.006

Figure Lengend Snippet: Down‐regulation of c‐Myc and NF‐κB resulted in significantly increased drug sensitivity. A) Louckes, B) BC3, and C) LCL1 cells were transduced with 3 specific lentiviral constructs producing shRNA sequences for control, ...

Article Snippet: The lentiviral vector HIV‐CS‐CG contains a GFP cassette under the CMV promoter ( Miyoshi et al., 1998 ), and Lentiviral packaging vector pCMV‐VSV‐G, pRSV‐Rev and pMDLg/pRRE were obtained from Addgene (Cambridge, MA).

Techniques: Transduction, Construct, shRNA